Fig. 8

Immunohistochemical analysis of neurodegeneration in the brains of ⍺-synuclein transgenic mice treated with anti-CD1d. (A) Representative bright field light microscopy images from the neocortex, hippocampus, and striatum of non-tg and ⍺-syn tg mice treated with IgG or anti-CD1d immunostained with an antibody against NeuN (neuronal cell marker). (B–D) Computer based image analysis showing estimated numbers of NeuN immunoreactivity cells in the neocortex and hippocampus of ⍺-syn tg mice and normalized levels with anti-CD1d treatment. (E) Representative bright field light microscopy images from the striatum and S. Nigra of non-tg and ⍺-syn tg mice treated with IgG and anti-CD1d immunostained with an antibody against TH (dopaminergic marker). (F, G) Computer based image analysis showing levels of TH immunoreactivity fibers in the striatum of IgG treated ⍺-syn tg mice and effect with anti-CD1d therapy. Estimates of TH positive cells in the S. Nigra are comparable among the four groups. Scale bars = 50 μm for A, 40 μm for E. n = 4 mice per group. Statistical significance determined by two-way ANOVA with post-hoc Tukey