Fig. 5
Fascin-1 deletion in microglia alters mechanical characterization after SCI. (A) Sagittal immunofluorescence images for the Cx43 (red) and DAPI (blue) from Fascin-1fl/fl and Fascin-1 CKO mice before SCI and at 7 and14 dpi. The lesion core was marked with the asterisks. Scale bar: 100 μm. (B) Percentage quantification of Cx43+ area. (C) Typical immunofluorescence images of CD68 (red) and DAPI (blue) in the penumbra region from two groups of mice as described above at 14 dpi. Scale bar: 20 μm. (D) Percentage quantification of CD68+ area in different sizes. (E) Sagittal immunofluorescence images of NeuN+ neurons (red) and GFAP+ astrocytes (green) in Z1-Z3 zones neighboring the central lesion core in two groups at 14 dpi. Scale bar: 200 μm. (F) Quantitative analysis of NeuN+ neurons. (G) The BMS score was used to evaluate locomotor function at 0, 1, 3, 7, and 14 dpi between Fascin-1fl/fl and Fascin-1 CKO groups. n = 3 animals in (B), (D), and (F). n = 8 animals in (G). ns, no significance; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by two-way ANOVA