Fig. 8
Reducing myosin activity rescues microglial migration and mechanical changes in Fascin-1 CKO mice. (A) Schematic of the Fascin-1fl/fl and Fascin-1 CKO mice treatment with Y-27,632 or vehicle. The myosin activity inhibitor Y-27,632 was administered by intraperitoneal injection daily from 4 h after injury until 14 dpi. (B) Sagittal immunofluorescence images of pMRLC (green), CX3CR1(red), and DAPI (blue) from Fascin-1fl/fl and Fascin-1 CKO mice treated with vehicle and Y-27,632 at 7 dpi. Scale bar: higher magnification, 20 μm; low magnification, 100 μm. (C) Percentage quantification of pMRLC+CX3CR1+ cells in relation to the total number of CX3CR1+ cells. (D) Sagittal immunofluorescence images of CX3CR1 (red) in different groups as described above at 7 and 14 dpi. Scale bar: 20 μm. (E, F) Quantification of the density of microglia in different distance from the lesion epicenter at 7 (E) and 14 dpi (F). (G, H) Sagittal immunofluorescence images for microglia (CX3CR1, red) and astrocyte (GFAP, green) in different groups as described above at 7 (G) and 14 dpi (H). Region a represents the GFAP− lesion core, and region b represents the GFAP+ penumbra region. Scale bar: 100 μm. (I, J) Comparison of the elastic properties of lesion core (a, I) and penumbra region (b, J) in different groups as described above in (I) and (J). The lesion core was marked with the asterisks. n = 3 animals in (C), (E), (F), (I), and (J). ***P < 0.001, ****P < 0.0001 by one-way ANOVA in (C). ****P < 0.0001 by two-way ANOVA in (E), (F), (I), and (J)