Fig. 9
Reducing myosin activity rescues inflammation, neuron survival, and functional recovery in Fascin-1 CKO mice. (A) Typical immunofluorescence images of CD68 (red) in the penumbra region in Fascin-1fl/fl mice treated with vehicle and Fascin-1 CKO mice treated with vehicle and Y-27,632 at 14 dpi. The nuclei were marked with DAPI (blue). Scale bar: 20 μm. (B) Reducing myosin activity rescues the size of CD68+ cells (red) in Fascin-1 CKO mice. (C) Sagittal immunofluorescence images of NeuN+ neurons (red) and GFAP+ astrocyte (green) in Z1-Z3 zones neighboring the central lesion core in three groups at 14 dpi. Scale bar: 200 μm. (D) Quantitative analysis of NeuN+ neurons. (E) The BMS score was used to assess locomotor function at 0, 1, 3, 7, and 14 dpi in three groups.n = 3 animals in (B) and (D). n = 8 animals in (E). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ####P < 0.0001 by two-way ANOVA