Fig. 6

Microglia in the contralateral dLGN still internalize VGLUT2 + synapses and C3 and display morphological features indicative of activation along with increased numbers at a chronic timepoint. A Representative microglial reconstructions with internalized VGLUT2 (green) and C3 (red), and microglial morphology (IBA1, magenta). Scale bar = 10 µm. B Microglia filament length to volume ratio in the contralateral dLGN. C, D Microglia count per 63 × high power field. Scale bar = 15 µm E Microglial internalization of C3 and F microglial internalization of VGLUT2 in the contralateral dLGN. B, E, F one-way ANOVA with Tukey correction for multiple comparisons. C t-test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Error bars = mean ± s.e.m