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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Neuroinflammation driven by human immunodeficiency virus-1 (HIV-1) directs the expression of long noncoding RNA RP11-677M14.2 resulting in dysregulation of neurogranin in vivo and in vitro

Fig. 6

HIV-1 infection causes Nrgn dysregulation in Brain organoids. (A) Schematic diagram of the experimental design is depicted. Primary adult brain microglia (0.5 × 106 cells) were infected with HIV-1 or mock-infected and were added to brain organoids for overnight. Microglia-embedded organoids were harvested at days 5 and 20 p.i. for RNA extraction, and at days 10 and 30 p.i. for immunostaining. (B) Mean of fold change variation in Nrgn, RP11-677M14.2 transcript and HIV-1 Gag expression compared to mock assessed through RT-qPCR (N = 3). Dotted lines represent the expression levels of mock-infected brain organoids. (C) Immunostaining of infected brain organoid for Nrgn (Cy5, red) and nuclei (blue) compared to mock-infected organoid. DAPI Scale bars show 10 μm, N = 3. The images are z-projections of image stacks acquired at ×40 magnification

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Journal of Neuroinflammation

ISSN: 1742-2094