Fig. 1

Expression profile of Mas and Ang-(1–7) after ICH. A Immunofluorescence assessment of Mas (green) in microglia/macrophages (iba1 + , red) in the sham group and the perihematomal area of ICH group. Nuclei were stained with DAPI (blue). Scale bar1 = 20 μm, scale bar2 = 10 μm. B Quantitative analyses of Iba1 + /Mas + microglia/macrophage and in the perihematomal area after ICH, ***P < 0.001 vs. ICH 1d, n = 3–6/group. C Comparison of perihematomal Ang-(1–7) levels between Sham and ICH mice at 3 days after ICH surgery. *P < 0.05 vs. Sham, n = 6/group. D Comparison of serum Ang-(1–7) levels between Sham and ICH mice at 3, 5, and 7 days after ICH surgery. **P < 0.01 vs. Sham; n = 10 in Sham and ICH-3d groups, n = 6 in ICH-5d and ICH-7d groups. E Representative images of colocalization of Mas with microglia (iba1, red) astrocytes (GFAP, red), neurons (NeuN, red) and vascular endothelial cell (CD31, red) in the perihematomal area of ICH (3 day) group. Nuclei were stained with DAPI (blue). Scale bar = 100 μm, n = 3/group. F The proportional expression of Mas in neurons, microglia and astrocytes surrounding the hematoma