Fig. 8

PLX5622-mediated microglia depletion attenuates the effect of AVE0991 in ICH mice. A Schematic diagram of PLX5622 use. B Representative immunofuorescence staining for Iba1 (green) in the perihematomal area after PLX5622 diet. Scale bar = 100 μm. C Quantification of Iba1 + microglia. ***P < 0.001 vs. Sham; ^###P < 0.001 vs. ICH, n = 6/group. D Representative images of the co-localization of TUNEL (green) with neurons (NeuN, red) in the perihematomal area at 3 days after ICH. Scale bar = 50 μm. E Quantitative analyses of TUNEL-positive cells in the perihematomal area at 3 days after ICH (n = 6/group). F Representative photograph of brain sections. G, H Quantitative analyses of hematoma volume and hemoglobin level at 3 days after ICH. Data was represented as mean ± SD. ***P < 0.001 vs Sham; n = 6/group. PLX, PLX5622; Veh, Vehicle; AVE, AVE0991