Fig. 2
From: Alpha-synuclein inclusion responsive microglia are resistant to CSF1R inhibition
Inclusion-associated increases in microglia persist in the SNpc despite CSF1R inhibition. A–D Ionized calcium binding adaptor molecule 1 (Iba1, green) and phosphorylated alpha synuclein at serine 129 (pSyn, red) immunofluorescence in the substantia nigra pars compacta (SNpc) 2 months post intrastriatal alpha-synuclein preformed fibril (α-syn PFF) or phosphate buffered saline (PBS) injection, with or without Pexidartinib (PLX3397B) treatment. E Quantitation of Iba1 immunoreactive microglia in the SNpc in all treatment groups. PFF injected rats display significantly more microglia in the SNpc in both chow treatment groups. PLX3397B treatment resulted in significant microglial depletion in both PBS and PFF rats (p ≤ 0.001). Black outline = no PLX3397B; green outline = PLX3397B; *p < 0.04; ***p = 0.0001; ****p < 0.0001. Values represent the mean ± SEM. Scale bars in A–D are 100 µm