Fig. 5
From: Alpha-synuclein inclusion responsive microglia are resistant to CSF1R inhibition
Expression of innate immune genes in microglia proximal to α-syn inclusions is maintained despite CSF1R inhibition. A Immunofluorescence (IF) for ionized calcium binding adaptor molecule 1 (Iba1, green) and fluorescent in-situ hybridization (FISH) for Cd74 (red) and Csf1r (blue). B IF for Iba1 (green) and FISH for Cd74 (red) and Cxcl10 (green). C IF for Iba1 (green) and FISH for Cd74 (red) and Fcer1g (green). D IF for Iba1 (green) and FISH for Cd74 (red) and Grn (green). E IF for Iba1 (green) and FISH for Cd74 (red) and Rt1-a2 (green). F IF for Iba1 (green) and FISH for Cd74 (red) and Tyrobp (green). All images taken in the ipsilateral SNpc 2 months post alpha-synuclein preformed fibril (α-syn PFF) injection with Pexidartinib (PLX3397B) treatment. Scale bars 50 µm