Fig. 6
From: Alpha-synuclein inclusion responsive microglia are resistant to CSF1R inhibition
Long term CSF1R inhibition results in significant microglial depletion during nigrostriatal degeneration. A–D Ionized calcium-binding adaptor molecule 1 (Iba1, green) and phosphorylated alpha-synuclein (pSyn, red) immunofluorescence in the substantia nigra pars compacta (SNpc) 6 months following intrastriatal alpha-synuclein preformed fibril (α-syn PFF) or phosphate buffered saline (PBS) injection, with or without Pexidartinib (PLX3397B). Modest accumulation of pSyn immunoreactive neurons in the ipsilateral SNpc is evident following α-syn PFF injection. E Quantitation of Iba1 immunoreactive microglia in the SNpc in all treatment groups. Six months of PLX3397B treatment resulted in significant microglial depletion in both PBS and PFF rats. α-syn PFF PLX3397B rats display significantly more microglia compared to PBS PLX3397B rats. Values represent the mean ± SEM. No PLX3397B = black outline, PLX3397B = green outline. ****p < 0.0001 ***p = 0.0001. Scale bars in A–D are 100 µm